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Phosphorus limitation might promote more toxin content in the marine invader dinoflagellate Alexandrium minutum ArchiMer
Maestrini, Serge; Bechemin, Christian; Grzebyk, Daniel; Hummert, Christian.
Alexandrium minutum (strain AM89BM) has been grown in semi-continuous culture (0.2 vol. d super(-1)) in N-limiting (NO sub(3) super(-): PO sub(4) super(3-)=1.6 and 3.16), in P-limiting (N: P=160 and 80), and in N and P balanced (N: P=16) media. The toxin content in cells changed greatly according to the N: P ratio. Cells grown in N: P balanced condition showed an average total paralytic shellfish poisoning (PSP) content of 1.24 fmol cell super(-1). In N-limiting conditions, cells contained ca. 3 times less toxin with mean values of 0.41-0.45 fmol cell super(-1). In contrast, cells grown in P-limiting conditions contained on average 3.5 and 7 times more toxins than in the balanced N: P condition: 4.31 fmol cell super(-1) in the N: P=160 medium and 8.01 fmol...
Tipo: Text Palavras-chave: Alexandrium minutum; Phosphorus limitation; Toxin content; PSP.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00173/28422/26753.pdf
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Réponses physiologiques et biochimiques à une limitation nutritive en phosphore ou en azote sur la réorientation métabolique des lipides polaires chez différentes espèces de microalgues marines ArchiMer
Huang, Bing.
Betaine lipids (BL) are P-free polar lipids, conversely to phospholipids (PL). The metabolic reorientation induced by phosphorus (P) limitation in microalgae producing BL (Tisochrysis lutea and Diacronema lutheri, Haptophyta) or producing low levels of BL (Phaeodactylum tricornutum, Bacillariophyta) was compared to that induced by nitrogen (N) limitation. The carbon destiny and flow in different biosynthetic pathways were studied with a multidisciplinary approach. P or N limitation differently affected carbon metabolism according to microalgal species. P limitation highly decreased photosynthetic activity and respiration of P. tricornutum and T. lutea. Consequently, carbon accumulation was higher than under N limitation in these two species. Both...
Tipo: Text Palavras-chave: Microalgues marines; Bétaïne lipides; Triacylglycérols; Limitation en phosphore; Lipides polaires; Acides gras oméga-3; Marine microalgae; Betaine lipids; Triacylglycerols; Phosphorus limitation; Polar lipids; Omega- 3 fatty acids.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00500/61169/64638.pdf
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